this book presents molecular biophysics, with a nuts and bolts approach, with the connection made from molecules to biological phenomena. the physical toolbox presented within this text will form a basis for students to enter into a wide range of pure and applied bioengineering fields in medical, food and pharmaceutical areas. the topics covered in the book include: a short introduction to the range of biological molecules typically encountered, mesoscopic forces that act between biological molecules, phase transitions that occur in biosystems (protein crystallisation, globule-coil transition etc), liquid crystallinity as an example of the delicate range of partially ordered phases found with biological molecules, how molecules move and propel themselves at the cellular level, the general features of self-assembly with examples from proteins, viruses and membranes, surface phenomena including adhesion, wetting and capillarity, models for polymeric dynamics and elasticity, charged ions, charged polymers, membranes, viscoelasticity/biorheology, force measurement, electrophoresis, rheology, motors, structural biomaterials, and the phase behaviour of dna.