This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression.Provides well-tested experimental protocols for each techniqueLists the reagents and preparation of each experiment separatelyContains a complete schedule of experiments and the preparation requiredIncludes study questions at the end of each chapter