The main objective of this research was to optimize the electron donor supply in sulphate reducing bioreactors treating sulphate rich wastewater. Two types of electron donor were tested: lactate and slow release electron donors such as carbohydrate based polymers and lignocellulosic biowastes. Biological sulphate reduction was evaluated in different bioreactor configurations: the inverse fluidized bed, sequencing batch and batch reactors. The reactors were tested under steady-state, high-rate and transient-state feeding conditions of electron donor and acceptor, respectively. The results showed that the inverse fluidized bed reactor configuration is robust and resilient to transient and high-rate feeding conditions at a hydraulic retention time as low as 0.125 d. The biological sulphate reduction was limited by the COD: sulphate ratio ( 82% either using carbohydrate based polymers or lignocellulosic bio-wastes, in batch bioreactors. The biological sulphate reduction was limited by the hydrolysis-fermentation rate and by the complexity of the slow release electron donors.