practical cell culture techniques focuses on the advantages of new tissue culture techniques that enable close and detailed study of many nerve cell types. neuroscience is one of the key areas whose rapid development has been driven by the proliferation of cell culture methodology; some areas of neuroscience, such as glial cell biology and function, now depend almost entirely on cell culture-based research projects. this latest volume of humanas renowned neuromethods series provides practical treatment of the new, powerful methodologies and results including: cell culture techniques specifically designed for the novice ? the environmental factors that play a role in tissue culturing ? cell markers ? nutrition requirements of the different cell types ? three-dimensional organotypic slice explant cultures ? neuronal cultures ? cultures of astrocytes, oligodendrocytes, cerebral capillary endothelium, and other cell types. boulton, baker, and walzs new volume on practical cell culture techniques is sure to be a major resource for everyone-whether expert or novice-involved in studying the central nervous system on the molecular level.