1. Top 5 Considerations When Choosing an Antibody Amy Archuleta and Michael D. Browning 2. Validating Antibody Specificities for Immunohistochemistry by Protein Blotting Biji T. Kurien and R. Hal Scofield 3. Recent Advances in Chromogens for Immunohistochemistry Bipin Gupta, George Yang, Olga Petrauskene, and Marc Key 4. Overview of Imaging Techniques in Immunohistochemistry Gerald Sedgewick 5. Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells Pravin J. Mishra and Debabrata Banerjee 6. Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments Hemant K. Mishra 7. Labeling of Phospho-Specific Antibodies with oYo-Link(R) Epitope Tags for Multiplex Immunostaining Jingwen Niu, Jodi Hagen, Feifan, Yu, Alexander E. Kalyuzhny, and Andrew Tsourkas 8. Multiplex Immunochromogenic Tissue Staining Employing Primary Antibodies from the Same Species Matthew Levin, Zohreh AkhavanAghdam, and David Schwartz 9. 3D Imaging for Cleared Tissues and Thicker Samples on Confocal and Light-Sheet Microscopes Sharla L. White, Amy T. Lam, and Hannah D. Buck 10. Optical Tissue Clearing Enables Three-Dimensional Morphometry in Experimental Nerve Regeneration Research Simeon C. Daeschler and Gregory H. Borschel 11. Protocol and Software for Automated Detection of Lysosome Active "Runs" and "Flights" with Wavelet Transform Approach Kristiana Kandere-Grzybowska, Konstantin Polev, Diana V. Kolygina, and Bartosz A. Grzybowski 12. Phenotyping of M1 and M2a Macrophages and Differential Expression of ACE-2 on Monocytes by Flow Cytometry: Impact of Cell Culture Conditions and Sample Processing Christine Goetz, Chris Hammerbeck, Kristina Boss, Ryan Peterson, and Jody Bonnevier 13. In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-Detection of Phosphorylated EGFR in A431 Cultured Cells Jodi Hagen, Ashley Oliver, and Alexander E. Kalyuzhny 14. In Situ Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-Detection of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue Ashley Oliver, Jodi Hagen, Sue Yang, and Alexander E. Kalyuzhny 15. Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry Wenfei Kang, Anthony Santella, Eric Rosiek, Maria Pulina, Eric Chan, Ning Fan, Murray J. Tipping, Afsar Barlas, Yevgeniy Romin, and Katia Manova-Todorova 16. Transcriptio