Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer ― from molecular methods to whole animal considerations ― for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems.Contains a comprehensive collection of transgenic animal and gene transfer methodsDiscusses background and introduction to techniques and animal systemsTeaches practical step-by-step protocolsFully revised with updates to reflect state-of-the-art technology and associated changes to date