Name: generation of cdna libraries: methods and protocols (en Inglés)
Price: 243520.00 CLP
Availability: InStock
Author: Ying, Shao-Yao
ISBN: 9781617373336

portada generation of cdna libraries: methods and protocols (en Inglés)

Formato

Libro Físico

Ilustrado por

Shao-Yao Ying

Editorial

Humana

Autor

Ying, Shao-Yao

Año

2010

Idioma

Inglés

N° páginas

338

Encuadernación

Tapa Blanda

Dimensiones

25.4 x 17.8 x 1.9 cm

Peso

0.61 kg.

ISBN

1617373338

ISBN13

9781617373336

Categorías

Anatomía
Génetica médica
Biología molecular

generation of cdna libraries: methods and protocols (en Inglés)

Shao-Yao Ying (Ilustrado por) · Humana · Tapa Blanda

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Reseña del libro "generation of cdna libraries: methods and protocols (en Inglés)"

Since its invention and subsequent development nearly 20 years ago, po- merase chain reaction (PCR) has been extensively utilized to identify numerous gene probes in vitro and in vivo. However, attempts to generate complete and full-length complementary cDNA libraries were, for the most part, fruitless and remained elusive until the last decade, when simple and rapid methods were developed. With current decoding and potential application of human genome information to genechips, there are urgent needs for identification of functional significance of these decoded gene sequences. Inherent in bringing these app- cations to fruition is the need to generate a complete and full-length cDNA library for potential functional assays of specific gene sequences. Generation of cDNA Libraries: Methods and Protocols serves as a laboratory manual on the evolution of generation of cDNA libraries, covering both ba- ground information and step-by-step practical laboratory recipes for which p- tocols, reagents, operational tips, instrumentation, and other requirements are detailed. The first chapter of the book is an overview of the basics of generating cDNA libraries, which include the following: (a) the definition of a cDNA library, (b) different kinds of cDNA libraries, (c) differences between methods for cDNA library generation using conventional approaches and novel stra- gies, including reverse generation of RNA repertoires from cDNA libraries, and (d) the quality of cDNA libraries.