This second edition volume provides an updated and comprehensive overview of miRNA target identification, spanning miRNA binding site prediction, integrative and network-based analyses, emerging technologies, and experimental validation strategies. Expanding on the first edition, this volume includes updated versions of several core chapters, as well as entirely new chapters that reflect major conceptual and technological advances in the field. The chapters in this book cover topics such as principles and evolving features of miRNA target recognition and prediction; systems-level and integrative analyses of miRNAs and their targets; emerging technologies and translational applications; and experimental validation of predicted miRNA targets. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, MicroRNA Target Identification: Methods and Protocols, Second Edition is a valuable resource for researchers working in computational biology, molecular biology, and translational medicine. This book inspires the continued evolution of the field and the increasing integration of computational and experimental perspectives in miRNA research.